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Image Search Results
Journal: Physiological Reports
Article Title: Effect of controlled hypotensive hemorrhage on plasma sodium levels in anesthetized pigs: An exploratory study
doi: 10.14814/phy2.15886
Figure Lengend Snippet: Schematic depicting experimental timeline, within timepoints running from baseline, which started at T1, (i.e., after instrumentation, preparation, and stabilization of the animals), followed by hemorrhage, and resuscitation and recovery, respectively. Euthanasia took place after T6. Within the first 15 min after T3, i.e., during resuscitation, hypovolemia was treated by replacing 60% of the total blood volume withdrawn during hemorrhage with retransfused blood and the remaining 40% with Ringer's acetate solution. Maintenance fluid therapy consisted of a half‐isotonic balanced crystalloid solution (70 mmol/L sodium, 45 mmol/L chloride, 25 mmol/L acetate in 2.5% glucose, Fresenius Kabi®). Blood draws were obtained at each timepoint. Plasma sodium, plasma potassium, plasma chloride, plasma lactate, blood hemoglobin, serum osmolality, cardiovascular parameters, renal blood flow, renal vascular resistance, renal oxygen delivery, and consumption data are presented at T1, T2, T3, and T6, whereas renin, aldosterone, and vasopressin‐neurophysin 2‐copeptin data, at T1, T3, and T6, respectively. Renal function, urine output, and electrolyte‐free water clearance were analyzed at the time intervals A, B, C, D, and E, respectively. Urinary excretion of aquaporin‐2 was analyzed at the time intervals A and E. Urine creatinine, sodium, potassium, and chloride concentration, and urine osmolality were analyzed from an aliquot from each time interval collection.
Article Snippet: Following washing as above, a rabbit polyclonal antibody directed against the
Techniques: Concentration Assay